Phytochemicals Screening, Proximate composition and Antioxidants Analysis of Italian Citrus paradisi Fruits

Authors

  • Ibrahim Iklimah Bandi Central Advanced Science Laboratory Complex, Usmanu Danfodiyo University, Sokoto, Nigeria Author
  • Abubakar Ibrahim Usmanu Danfodiyo University, Sokoto Author https://orcid.org/0000-0002-8037-9406
  • Ibrahim Shehu Department of Chemistry, Shehu Shagari University of Education, Sokoto, Nigeria Author
  • Adiya Zainab Sarkin Gobir Department of Pure and Environmental Chemistry, Usmanu Danfodiyo University, Sokoto, Nigeria Author
  • Buhari Hafsat Bature Department of Pure and Environmental Chemistry, Usmanu Danfodiyo University, Sokoto, Nigeria Author
  • Shehu Samira Rara Central Advanced Science Laboratory Complex, Usmanu Danfodiyo University, Sokoto, Nigeria & Department of Pure and Environmental Chemistry, Usmanu Danfodiyo University, Sokoto, Nigeria Author

DOI:

https://doi.org/10.30872/j.trop.pharm.chem.v8i1.162

Keywords:

Antioxidant, Citrus paradise, Nutrients, Phytochemicals, Proximate composition

Abstract

Citrus paradise is a largely consumed fruit for nutrition and medicinal importance. This study was conducted to evaluate the phytochemicals, proximate, and antioxidants contents of Italian Citrus paradisi fruits. The phytochemicals screening and proximate analysis were performed using the methods of AOAC. Antioxidants contents and capacities of the extract were determined by DPPH and FRAP Assay. The grapefruits aqueous extract revealed the presence of various phytochemicals and contains significant (< 0.05) amount of carbohydrates (89.36 %), moisture (74.80 %), lipids (4.56 %), protein (3.36 %), crude fiber (2.36 %), and ash content (0.83 %). The extract exhibited significant (< 0.05) increase in DPPH percentage inhibition of free radical scavenging activity and FRAP properties comparable to that of the standard, ascorbic acid. The grapefruits extract contains various phytochemicals, proximate nutrients, and exhibited free radical scavenging activity. The grapefruits have nutritional status, antioxidant properties, and medicinal importance.

References

Abubakar I., Aliyu J. D., Abdullahi Z., Zubairu Z., Umar A. S., Ahmad F. 2022. Phytochemical Screening, Nutritional and Anti-nutritional Composition of Aqueous Rhizome Extract of Curcuma longa, IOSR JBB, Vol. 8 (2), 1-9.

Gülçin, I. 2012. Antioxidant activity of food constituents: An overview, Arch. Toxicol., Vol. 86, 345–391.

Ferguson, L. R. 2010. Chronic inflammation and mutagenesis. Mutat. Res. Fundam. Mol. Mech., Vol. 690, 3–11.

Rabha A, Wangchu L, Singh B. 2013. Studies on genetic diversity of citrus in east Siang district of Arunachal Pradesh, International Journal Agriculture and Environmental Biotechnology, Vol. 6, 131-37.

Manthey J. A. 2044. Fractionation of orange peel phenols in ultra-filtered molasses and balance studies of their antioxidant levels. Journal of Agriculture and Food Chemistry, Vol. 52, 7586-7592.

Pandharipande S, Makode H. 2012. Separation of oil and pectin from orange peel and study of effect of pH of extracting medium on the yield of pectin, Journal of Engineering Research and Studies, Vol. 3 (2), 6–9.

Kamal G. M., Anwar F., Hussain A. l., Sarri N., Ashraf M. Y. 2012. Yield and chemical composition of Citrus essential oils as affected by drying pretreatment of peels, International Food Research Journal, Vol. 18 (4), 1275-1282.

FAO, 2004. FAOSTAT data 2005. Food and Agricultural Organisation of the United Nations, 00100, Rome, Italy. February, 2005.

Silalhi J. 2002. Anticancer and health protective properties of Citrus fruit components, Asia Pacific Journal of Clinical Nutrition, Vol. 11, 79-84.

Saidani M., Dhifi W., Marzouk B. 2004. Lipid evaluation of some Tunisian Citrus seeds, Journal of Food Lipids, Vol. l (11), 242-250.

Guimaraes R., Barros L., Barreira J. C., Sousa M. J., Carvalho A. M. 2010. Ferreira ICGR. Targeting excessive free radicals with peels and juices of citrus fruits: grapefruit, lemon, lime and orange, Food and Chemical Toxicology, Vol. 48 (1), 99–106.

Atolani O., Omere J., Otuechere C. A., Adewuyi A. 2012. Antioxidant and cytotoxicity effects of seed oils from edible fruits, Journal of Acute, 130-134.

Kamran G., Youcef G., Ebrahimzadeh M. A. 2009. Antioxidant activity, phenol and flavonoid contents of 13 citrus species peels and tissues, Pakistan Journal of Pharmaceutical Sciences, Vol. 22(3), 277-281.

Baghurst K. 2003. The health benefits of citrus fruits, CSIRO Health Sciences and Nutrition full report, Project No: CT02057, Horticulture Australia.

Lipson S. M., Sethi L., Cohen P., Gordon R. E., Tan I. P., Burdowski A., Stotzky G. 2007. Antiviral effects on bacteriophages and rotavirus by cranberry juice, Phytomedicine, Vol. 14(1), 23-30.

Zoumas-Morse C., Rock C. L., Quintana E. L., Neuhouser M. L., Gerner E. W., Meyskens F. L. 2007. Development of a polyamine database for assessing dietary intake, Journal American Diet Association, Vol. 107(6), 1024-1027.

Srividhya M., Ramanatha K., Krishnanand N. 2013. Efficacy of citrus fruit peel extracts against pathogens causing gastrointestinal disorders, International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 5(4), 160-163.

Silver H. J., Dietrich M. S., Niswender K. D. 2011. Effects of grapefruit, grapefruit juice and water preloads on energy balance, weight loss, body composition, and cardio metabolic risk in free-living obese adults, Nutrition Metabolism, Vol. 8, 1-8.

Kiani J., Imam S. Z. 2007. Medicinal importance of grapefruit juice and its interaction with various drugs, Nutrition Journal, Vol. 6, 33.

Tundis R., Loizzo M. R., Menichini F. 2014. An overview on chemical aspects and potential health benefits of limonoids and their derivatives, Crit. Rev. Food Sci. Nutr., Vol. 54, 225–250.

Zou Z., Xi W., Hu Y., Nie, C. 2015. Zhou Z. Antioxidant activity of citrus fruits, Food Chemistry, Vol. 196, 885–896.

Platt R. 2000. Current concepts in optimum nutrition for cardio vascular disease. Prev Cardiol., Vol. 3, 83-87.

Chidambara K., Murthy K. N., Jayaprakasha G. K., Kumar V., Rathore K. S., Patil B. S. 2011. Citrus limonin and its glucoside inhibit colon adenocarcinoma cell proliferation through apoptosis. Jounal of Agriculture and Food Chemistry, Vol. 59, 2314-2323.

Okunowo W, Oyedeji O, Afolabi L, Matanmi E. 2013. Essential oil of grapefruit (Citrus paradisi) peels and its antimicrobial activities. Amr. J. Plant Sci., Vol. 4, 1-9.

Mosa E. O., Elhadi M. A., Mahgoub S. E. 2012. Preliminary phytochemical evaluation and seed proximate analysis of Surib (Sesbanialeptocarpa DC.) SJMS. Vol. 7 (4), 29-34.

Abubakar I, Muhammad H. Y, Shuaibu Y. B, Abubakar M. G. 2020. Anti-ulcer activity of methanol extract of the leaves of Hannoa klaineana in rats. Journal of Phytopharmacology, Vol. 9 (4), 258-264.

Trease G. E., Evans W. C. 1989. Pharmacognosy. 13th Edition, Bailere Traiadal, London, p.69.

Harborne J. B. 1973. Phytochemical methods: A guide to modern Techniques of plant Analysis. Chapman and Hall Ltd, London. pp. 279.

El-Olemyl M. M., Fraid J. A., Abdulfattah A. A. 1994. Experimental photochemistry. A laboratory manual Afifi, Abdel Fattah, A comp., IV King Saud university press, UK, pp 1-134.

Evans W. C. 1996. Commerce and production: principles related to the commercial production, quality and standardization of natural products. In: Trease GE & Evans WC (Editors), Pharmacognosy. 14th edn. Saunders, London.

AOAC. 1999. Official Methods of Analysis, 15th edn. Association of Official Analytical Chemists, Arlington, VA.

AOAC 2010. Official Methods. Minerals, Official Methods of analysis, Washington, DC, USA. AOAC.

Singleton V. L., Orthofer R., Lamuela-Raventós R. M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymology, Vol. 299, 152-178.

Abubakar I., Muhammad H. Y., Shuaibu Y. B., Abubakar M. G., Hassana S. W. 2021. Anti-ulcerogenic activity of the fractions of methanol leaves extract of Hannoa Klaineana in Wistar rats, International Journal of Pharma and Biosciences, Vol. 12(2), 27-40.

Lamaison J. L. C., Carret A. 1990. Teneursenprincipaux flavonoids des Feurs de CrataegusmonogynaJacqet de Cratae- guslaevigata (Piret DC) enfonction de la vegetation, Plantesmed Phytother, Vol. 25, 12-16.

Gyamfi M. A., Yonamine M., Aniya Y. 1999. Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguine on experimentally induced liver injuries. Gen Pharmacol., Vol. 32 (6), 661-667.

Benzie I. F. F., Strain J. J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay, Analytical Biochemistry, Vol. 239, 70-76.

Oghenejobo M., Opajobi O. A., Bethel O. U. S. 2017. Antibacterial evaluation, phytochemical screening and ascorbic acid assay of turmeric (Curcuma longa), MOJ Bioequiv Availab., Vol. 4 (2), 232-239.

Brewer M. S. 2011. Natural antioxidants: Sources, compounds, mechanism of action and potential applications, Comparative Reviews in Food Science and Food Safety, Vol. 10 (4), 221-247.

Mamta S., Jyoti S., Rajeev N., Dharmendra S., Abhishek G. 2013. Phytochemistry of medicinal plants, Journal of Pharmacognosy and Phytochemistry, Vol. 1 (6), 168-182.

Majeed M., Vladimir B., Murray F. 2004. Turmeric and the healing curcuminoids: Their amazing antioxidant properties and protective powers, New Canaan CT: Keats Pub.

Kar A. 2007. Pharmacognosy and Pharmabiotechnology (Revised- Expanded Second Edition), New Age International Limited Publishers, New Delhi, pp 332-360.

Wadhave A. A., Jadhav A. I., Arsul V. A. 2014. Plant proteins applications: A review. WJPPS, Vol. 3 (3), 702-712.

Lattimer J. M., Haub M. D. 2010. Effects of Dietary Fiber and Its Components on Metabolic Health. Review, Nutrients, Vol. 2, 1266-1289.

Onwuka G. l. 2005. Food analysis and instrumentation. Theory and practice. 1st edition, Naphthali Prints Nigeria, pp 1-129.

Okwu D. E. 2004. Phytochemicals and Vitamin content of indigenous spicies of South Eastern Nigeria, Journal of Sustain Agriculture and Environment, Vol. 6, 30-34.

Steenkamp V., Stewart M. J., Chimuka L., Cukrowska E. 2005. Uranium concentration in South African herbal remedies, Health physiology, Vol. 89, 79-83.

Adedosu O. T., Adekunle A. S., Adedeji A. L., Afolabi O. K., Oyedeji T. A. 2013. Antioxidant and anti-lipid peroxidation potentials of the ethylacetate and chloroform extracts of Basella alba leaves, Asian Journal of National and Applied Sciences, Vol. 2, 81-88.

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Published

2024-06-30

How to Cite

Phytochemicals Screening, Proximate composition and Antioxidants Analysis of Italian Citrus paradisi Fruits. (2024). Journal of Tropical Pharmacy and Chemistry , 8(1), 17–27. https://doi.org/10.30872/j.trop.pharm.chem.v8i1.162