Antidiabetic Activities of Broccoli Extracts (Brassica oleracea L.var italica) on Mice Induced Streptozotocin-Nicotinamide
DOI:
https://doi.org/10.30872/j.trop.pharm.chem.v4i4.269Keywords:
antidiabetic activities, broccoli extract, streptozotocin-nicotinamideAbstract
Several plant species have been used for the treatment of diabetes. Research on plants that can be used to treat diabetes such as Cinnamomum cassia and Cinnamomum zeylanicum, Piper sarmentosum Roxb, Nelumbo nucifera, Ceiba petandra Memecylon malabaricum. Broccoli (Brassica oleracea L var italics) is a flower of vegetables like cabbage plants. Broccoli juice is made capable of direct and indirect effects on the reduction of blood LDL; the indirect effect is by repairing pancreatic beta cells and increasing insulin sensitivity. The purpose of this study was to determine whether broccoli has activity as antidiabetic. The subjects consisted of 15 mice induced with nicotinamide 70 mg/kg BW and Stz 150 mg/kg BW. Diabetic mice were divided into the positive control group (glibenclamide), negative control group (CMC Na) and three treatment groups. The test material is broccoli extract dose 75 mg / kg BW, 150 mg /kg BW and 300 mg/kg BW given peroral for 14 days. Anova test results showed that there was a significant difference in the average percentage of decreased blood glucose levels in all treatments. The result of Post Hock Tukey HSD test with a 95% confidence level showed a significant difference in the negative control group compared with positive control group and extract 300mg / kg BW. This shows that positive control group and extracts 300mg / kg body weight have an activity to decrease blood glucose level.
References
Pramono, S., 2002. Kontribusi bahan obat alam dalam mengatasi krisis bahan obat di Indonesia. Jurnal Bahan Alam Indonesia. 1. 18-20.
Sari, L. O. R. K., 2012. Pemanfaatan obat tradisional dengan pertimbangan manfaat dan keamanannya. Pharmaceutical Sciences and Research (PSR). 3. 1-7.
Verspohl, E. J., Bauer, K., Neddermann, E., 2005. Antidiabetic effect of Cinnamomum cassia and Cinnamomum zeylanicum in vivo and in vitro. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 19. 203-206.
Peungvicha, P., Thirawarapan, S. S., Temsiririrkkul, R., Watanabe, H., Prasain, J. K., Kadota, S., 1998. Hypoglycemic effect of the water extract of Piper sarmentosum in rats. Journal of ethnopharmacology. 60. 27-32.
Mukherjee, P. K., Saha, K., Pal, M., Saha, B., 1997. Effect of Nelumbo nucifera rhizome extract on blood sugar level in rats. Journal of ethnopharmacology. 58. 207-213.
Elekofehinti, O. O., 2015. Saponins: Anti-diabetic principles from medicinal plants–A review. Pathophysiology. 22. 95-103.
Amalraj, T., Ignacimuthu, S., 1998. Evaluation of the hypoglycaemic effect of Memecylon umbellatum in normal and alloxan diabetic mice. Journal of ethnopharmacology. 62. 247-250.
Setyoadi, S., Utami, Y. W., Yuliatun, L., 2014. Jus brokoli menurunkan kadar low density lipoprotein darah pada tikus model diabetes melitus. Jurnal Kedokteran Brawijaya. 28. 26-29.
Bahadoran, Z., Tohidi, M., Nazeri, P., Mehran, M., Azizi, F., Mirmiran, P., 2012. Effect of broccoli sprouts on insulin resistance in type 2 diabetic patients: a randomized double-blind clinical trial. International journal of food sciences and nutrition. 63. 767-771.
Shah, M. A., Sarker, M., Gousuddin, M., 2016. Antidiabetic potential of Brassica Oleracea Var. Italica in type 2 diabetic sprague dawley (sd) rats. Int J Pharmacogn Phytochem Res. 8. 462-469.
Antidiabetic Activities of Broccoli Extracts (Brassica oleraceaL.var italica) on Mice Induced Streptozotocin-NicotinamideJ. Trop. Pharm. Chem. 2018. Vol 4. No. 4.p-ISSN: 2087-7099; e-ISSN: 2407-6090188
Wulandari, N. E., Wirawanni, Y., 2014. Pengaruh Pemberian Brokoli Kukus (Brassica Oleracea) Terhadap Kadar Glukosa Darah Puasa Wanita Prediabetes. Diponegoro University.
Kaneto, H., Kajimoto, Y., Miyagawa, J.-i., Matsuoka, T.-a., Fujitani, Y., Umayahara, Y., Hanafusa, T., Matsuzawa, Y., Yamasaki, Y., Hori, M., 1999. Beneficial effects of antioxidants in diabetes: possible protection of pancreatic beta-cells against glucosetoxicity. Diabetes. 48. 2398-2406.
Indrawati, S., Yuliet, Y., Ihwan, I., 2015. Efek Antidiabetes Ekstrak Air Kulit Buah Pisang Ambon (Musa paradisiacaL.) terhadap Mencit (Mus musculus) Model Hiperglikemia. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy). 1. 133-140.
Perlitasari, Y., Prasetyo, D. H., 2016. Pengaruh pemberian ekstrak herba anting-anting (Acalypha indica) terhadap kadar malondialdehyde (MDA) pada mencit Balb/C induksi streptozotocin. Bioteknologi. 13. 83-90.
Sangi, M., Runtuwene, M. R., Simbala, H. E., Makang, V. M., 2008. Analisis fitokimia tumbuhan obat di Kabupaten Minahasa Utara. Chemistry Progress. 1. 47-53.
Aniszewki, T., 2007. Alkaloid-secrets of life. Belanda: Elsevier.
Bahadoran, Z., Mirmiran, P.,Hosseinpanah, F., Hedayati, M., Hosseinpour-Niazi, S., Azizi, F., 2011. Broccoli sprouts reduce oxidative stress in type 2 diabetes: a randomized double-blind clinical trial. European journal of clinical nutrition. 65. 972.
Rao, Α., Gurfinkel, D., 2000. The bioactivity of saponins: triterpenoid and steroidal glycosides. Drug metabolism and drug interactions. 17. 211-236.
Kumari, M., Jain, S., 2012. Tannins: An antinutrient with positive effect to manage diabetes. Research Journal of Recent Sciences. 2277. 2502.
Patel, V., Sharma, V., 2014. Effect of Brassica oleracea Extracts on Blood Glucose and Antioxidant Profile in Streptozotocin Induced Diabetic Rats. J. Med. Pharm. Innov. 1. 4-9.
Grassi, D., Desideri, G., Ferri, C., 2010. Flavonoids: antioxidants against atherosclerosis. Nutrients. 2. 889-902.
Epand, R. F., Savage, P. B., Epand, R. M., 2007. Bacterial lipid composition and the antimicrobial efficacy of cationic steroid compounds (Ceragenins). Biochimica et Biophysica Acta (BBA)-Biomembranes. 1768. 2500-2509.
Fatharanni, M. O., Anggraini, D. I., 2017. Efektivitas Brokoli (Brassica Oleracea var. Italica) dalam Menurunkan Kadar Kolesterol Total pada Penderita Obesitas. Jurnal Majority. 6. 64-70.
