Bacterial Symbionts of Soft Coral Lobophytum Sp. From Panjang Island, Jepara, Indonesia With Antimicrobial MDR TB Potency
DOI:
https://doi.org/10.30872/j.trop.pharm.chem.v4i5.272Keywords:
bacterial symbiont, Lobophytum sp., MDR TB, Virgibacillus marismortuiAbstract
Tubercolusis is a disease that attacks the lungs. The disease is caused by the bacterium mycobacterium tubercolusis. The bacteria mycobacterium tubercolusis can be killed by antibiotics. However, continuous use of antibiotics can cause bacterial resistance. So we need to find new drugs that can prevent multi-drug resistant tuberculosis. This study aims to determine the antibacterial activity of MDR TB from soft coral symbiont bacteria Lobophytum sp. There were 6 bacterial isolates obtained from soft coral Lobophytum sp. One isolate from Lobophytum-associated bacteria were successfully screened for antimycobacterial against MDR TB bacteria. PLO2 was found to inhibit the growth of MDR TB (MDR TB strain SIRE and R). Based on the results of identification with PCR, soft coral symbionts of PLO2 was closely related to Virgibacillus marismortui with homology of 99%.
References
York, T., De Wet, H., Van Vuuren, S., 2011. Plants used for treating respiratory infections in rural Maputaland, KwaZulu-Natal, South Africa. Journal of ethnopharmacology. 135. 696-710.
Chen, Y.-L., Huang, S.-T., Sun, F.-M., Chiang, Y.-L., Chiang, C.-J., Tsai, C.-M., Weng, C.-J., 2011. Transformation of cinnamic acid from trans-to cis-form raises a notable bactericidal and synergistic activity against multiple-drug resistant Mycobacterium tuberculosis. European Journal of Pharmaceutical Sciences. 43. 188-194.
Faulkner, D. J., 2000. Marine pharmacology. Antonie van leeuwenhoek. 77. 135-145.
Piel, J., 2006. Bacterial symbionts: prospects for the sustainable production of invertebrate-derived pharmaceuticals. Current medicinal chemistry. 13. 39-50.
Muscholl-Silberhorn, A., Thiel, V., Imhoff, J. F., 2008. Abundance and bioactivity of cultured sponge-associated bacteria from the Mediterranean Sea. Microbial Ecology. 55. 94-106.
Xi, L., Ruan, J., Huang, Y., 2012. Diversity and biosynthetic potential P. Lo 2 Virgibacillus marismortui Vulcanibacillus modesticaldus Vulcanibacillus sp. Bacillus subterraneus Bacillus kyonggiensis Bacillus gottheilii Bacterial Symbionts of Soft Coral Lobophytum Sp. From Panjang Island, Jepara, Indonesia With Antimicrobial MDR TB PotencyJ. Trop. Pharm. Chem. 2019. Vol 4. No. 5.p-ISSN: 2087-7099; e-ISSN: 2407-6090201of culturable actinomycetes associated with marine sponges in the China seas. International journal of molecular sciences. 13. 5917-5932.
Zeng, Z., Zhao, J., Ke, C., Wang, D., 2013. Antimicrobial activities of novel cultivable bacteria isolated from marine sponge Tedania anhelans. Chinese journal of oceanology and limnology. 31. 581-590.
Lee, Y. K., Lee, J.-H., Lee, H. K., 2001. Microbial symbiosis in marine sponges. JOURNAL OF MICROBIOLOGY-SEOUL-. 39. 254-264.[9]Thiel, V., 2006. Sponge-associated bacteria: specificity, diversity and antimicrobial potential. Christian-Albrechts-Universität.
Madigan, M. T., Martinko, J. M., Parker, J., 1997. Brock biology of microorganisms.Prentice hall Upper Saddle River, NJ.
Sulistiyani, S., Nugraheni, S. A., Radjasa, O. K., Sabdono, A., Khoeri, M. M., 2010. Antibacterial activities of bacterial symbionts of soft coral Sinularia sp. against tuberculosis bacteria. Journal of Coastal Development. 14. 45-50.[12]Kusmita, L., Mutiara, E. V., Nuryadi, H., Pratama, P. A., Wiguna, A. S., Radjasa, O. K., 2017. Characterization of carotenoid pigments from bacterial symbionts of soft-coral Sarcophyton sp. from North Java Sea. International Aquatic Research. 9. 61-69.
Khan, F. A., Hussain, I., Farooq, S., Ahmad, M., Arif, M., Rehman, I. U., 2011. Phytochemical screening of some Pakistanian medicinal plants. Middle-East J Sci Res. 8. 575-578.
Yadav, R., Agarwala, M., 2011. Phytochemical analysis of some medicinal plants. Journal of phytology.
Khayyat, S., Al-Kattan, M. O., 2017. Phytochemical screening and antimicrobial activities of Costus speciosus and Sea Qust. Biomedical Research. 28.
Walsh, P. S., Metzger, D. A., Higuchi, R., 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques. 10. 506-513.
Long, R. A., Azam, F., 2001. Antagonistic interactions among marine pelagic bacteria. Applied and environmental microbiology. 67. 4975-4983.
Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W., Lipman, D. J., 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic acids research. 25. 3389-3402.
Radjasa, O. K., Khoeri, M. M., Darusallam, C. C., Trimasanto, H., Sudoyo, H., 2013. Bacterial symbionts of reef invertebrates: screening for anti-pathogenic bacteria activity. Biodiversity. 14. 80-86.
Putra, M. Y., Murniasih, T., Swasono, R. T., Wibowo, J. T., Saputri, A. N. C., Widhiana, M. R., Arlyza, I. S., 2016. Secondary metabolites and their biological activities in Indonesian soft coral of the genus Lobophytum. Asian Pacific Journal of Tropical Biomedicine. 6. 909-913.
Effendi, H., Soedharma, D., Kawaroe, M., Subhan, B., Arafat, D., Trisyulianti I. 2016. Potential bioactivity of artificially fragmented soft coral Sinularia sp.
Bacterial Symbionts of Soft Coral Lobophytum Sp. From Panjang Island, Jepara, Indonesia With Antimicrobial MDR TB PotencyJ. Trop. Pharm. Chem. 2019. Vol 4. No. 5.p-ISSN: 2087-7099; e-ISSN: 2407-6090202and Lobophytum sp. transplantation. International Journal of Pharmaceutical and Clinical Research. 8(5): 481–484.
Bahry, M. S., Pringgenies, D. and Trianto, A. 2016. Molecular Identification of Marine Symbiont Bacteria of Gastropods from the Waters of the Krakal Coast Yogyakarta and Its Potential as a Multi-drug Resistant (MDR) Antibacterial Agent.International Symposium on Applied Chemistry (ISAC).
Romanenko, L. A. Uchino, M., Kalinovskaya, N. I., Mikhailova, V. V. 2008. Isolation, phylogenetic analysis and screening of marine mollusc-associated bacteria for antimicrobial, hemolytic and surface activities.Microbiological Research.163(6): 633–644.[24] Murti, P. D. B. and Radjasa, O. K. 2012. Antibacterial Activity Of Bacterial Symbionts Of Soft Coral Lobophytumsp. Against MDR Bacteria Escherichia coliand Staphyllococcus aureus. Journal of Coastal Development.15(3): 297–302.
Sulistiyani, Wahjono, H., Radjasa, O. K., Sabdono, A., Khoeri, M. M., Karyana, E. 2015. Antimycobacterial Activities from Seagrass Enhalus sp. Associated Bacteria Against Multi Drug Resistance Tuberculosis (MDR TB) Bacteria. Procedia Environmental Sciences.23: 253–259.
Burgess, J. G.,Boyd, K., Armstrong, E., Jiang, Z., Yan, L., Berggren, M., May, U., Pisacane, T., Granmo, A., Adams, D. R.2003. The Development of a Marine Natural Product-based Antifouling Paint The Development of a Marine Natural Product-based Antifouling Paint. Biofouling. 19: 197 –205.
Cowan, M.1999. Plant Productas Antimicrobial Agent. Clinical Microbiology Review.12 (4):564-582.
Hagström, A., J.U.L. 2000. Pinhassi and Zweifel. Biogeographical Diversity Among Marine Bacterioplankton. Aquat. Microb. Ecol.21 : 231-244.
da Silva M. A. C., Cavalett, A., Spinner, A., Rosa, D. C., Jasper, R. B., Quecine, M. C., Bonatelli, M. L., Pizzirani-Kleiner, A., Corção, G., Lima, A. O. S. 2013. Phylogenetic identification of marine bacteria isolated from deep-sea sediments of the eastern South Atlantic Ocean. SpringerPlus.2: 1–10. doi: 10.1186/2193-1801-2-127.
Arahal, D. R., Ma ́rquez, M. C., Volcani, B. E., Schleifer, K. H. & Ventosa, A. 1999. Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea. Int J Syst Bacteriol.49: 521–530.
Heyrman, L., Logan, N. A., Busse, H., Balcaen, A., Lebbe, L.,Rodriguez-Diaz, M., Swings, J., Vos P. D.2003. Virgibacillus carmonensissp. nov., Virgibacillus necropolissp. nov. and Virgibacillus picturaesp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus salibacillus to Virgibacillus, as Virgibacillus marismortuicomb. nov. and Virgibacillus salexigenscomb. nov., and emended description of the genus Virgibacillus.Int. J. Syst. Evol. Microbiol.53:501–511.
