Potensi Antimikroba Fraksi Aktif Ekstrak n-Heksan Daun Rami (B.virgata F.) Guill terhadap Beberapa Mikroba Uji
Keywords:
leaf B. virgata, antimicrobial, fraction soluble extracts of n-hexane, solid dilutionAbstract
This study aims to determine the potential antimicrobial activity of extracts soluble, insoluble n-hexane and fractions of n-hexane extract of leaves of B. virgata, some microbial testing with solid dilution method. Test material used was n-hexane-soluble extract (A) and hexane-insoluble (B), two extract was monitored by Thin Layer Chromatography method (TLC) chemical components, then tested its activity by solid dilution method. Test results of the antimicrobial activity of both types of extracts showed better activity than the methanol extract of soluble fraction of n-hexane and methanol extract of insoluble, because it can inhibit the growth of the bacterium E. coli, V. cholerae and S. thyposa, while the methanol extract of n-hexane soluble not only able to inhibit the bacteria V. cholerae, at a concentration of 1000 μg/ml medium. Fraction of methanol extract of n-hexane soluble (A) is then fractionated by the method of three funel system using eluent hexane: methanol: water (20: 6: 0.5), obtained three kinds of fractions ie fraction A, fraction B and fraction C. The antibacterial activity tes result of fraction A is active against bacteria V. chilerae, B fraction active against bacteria V.cholerae, S. thyposa and E. coli, and the active fractions of the yeast C.albicans C respectively each active at a concentration of 750 μg/ml medium.
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