Uji Sitotoksik dan Uji Kombinasi Fraksi Etil Asetat Ekstrak Etanol Akar Pasak Bumi (Eurycoma longifolia Jack.,) dan Doksorubisin pada Sel Limfosit
Keywords:
Pasak bumi roots, doxorubicin, Cytotoxicity test, combination test, lymphocyte cellsAbstract
The cytotoxicity and combination test of ethyl acetate fraction of ethanolic extract of Pasak bumi roots (Eurycoma longifolia Jack.) and doxorubicin have been made to the normal lymphocyte cells in vitro. The tests were carried out by using MTT method. Principle of the MTT method is to measure the mitochondrial dehydrogenase activity in living cells that have the ability to convert MTT into formazan. Cytotoxicity test for fraction performed at concentrations of 2000;1000;500;250;125;62,5;31,25 μg/mL and the concentrations of doxorubicin at 4;2;1;0,5;0,25;0,125;0,0625 μg/mL. From the tests IC50 values obtained ethyl acetate fraction of ethanolic extract of pasak bumi roots and doxorubicin against lymphocyte cells each 44 μg/mL and 1.1 μg/mL. The results of combination index (CI) value is 73,282 (CI>1= antagonist) at concentration of the combination 22 μg/mL for ethyl acetate fraction and 0,5547 for doxorubicin. The Results showed ethyl acetate fraction of ethanolic extract of the pasak bumi roots has lower toxicity than doxorubicin and it can be used for the combination chemotherapy with doxorubicin.
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