Aktivitas Hepatoprotektor Batang Fibraure tinctoria Lour Secara In Vivo
Keywords:
Fibraurea tinctoria, hepatoprotector, in vivoAbstract
Study on Fibraurea tinctoria Lour (FT) stems gives information about its traditional utility as yellow fever treatment. Research of antecedent of FT stem proved that inhibited lipid peroxidation more effective than tocopherol acetate. These study was intended to prove hepatoprotector activity of ethanolic FT stem extract by in vivo. FT stem extract was macerated using absolute ethanol during 5 days that was repeated 3 times . FT stem extract hepatoprotector activity by in vivo was tested using carbon tetrachloride induced hepatotoxicity on Wistar rat. They were given FT stem extract orally once a day at dose 50, 100, and 200 mg/kgBW and Curcumin at dose 50 mg/KgBW as positive control. After 10 days, all groups were examined liver function (SGOT, SGPT, ALP), liver Malonedialdehide (MDA) level by Thiobarbituric acid method, and liver histopathology by Haemotoxylin-Eosin staining. Group that induced by CCl4 showed significant elevation of SGOT, SGPT and ALT also Liver MDA than group control. FT stem extract treatment inhibited elevation of SGOT, SGPT, ALT and Liver MDA significantly.Qualitative histopathological examination on Group 2 showed extensive fibrosis and necrosis, along with periportal PMN and lymphocyte infiltration. FT stem extract treatment inhibited pathological change that was induced by CCl4. Dose elevation showed tendency of stronger inhibition on liver cell tissue destruction and inflammation.
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