Isolation and Characterization Endophytic Fungal Isolate from Peronema canescens Jack Leaf and Coptosapelta tomentosa Val. K. Heyne Root
DOI:
https://doi.org/10.30872/j.trop.pharm.chem.v4i5.275Abstract
Has been done Isolation, Characterization and Secondary Metabolite Endophytic Fungal Isolate from Peronema canescens Jack Leave and Coptosapelta tomentosa Valeton K. Heyne Root. The aim of this research is to know the number of fungal isolates, chromatogram profile and secondary metabolite group of endophytic fungal isolates from P. canencens leaves and C. tomentosa root. Characterization of endophytic fungal isolates was done macroscopically and microscopically. Identification of secondary metabolites endophytic fungal isolates were performed by chemical reaction test and TLC (Thin Layer Chromatography) method with specific spray reagents. The data of this study were obtained based on the number of endophytic fungal that can be isolated, observing macroscopic and microscopic morphological profiles, chromatogram profile and secondary metabolites of each endophytic fungal isolated. The results showed that endophytic fungal that can be isolated from P. canencens leaves four isolates, and two isolates from C. tomentosa root. Morphological profile macroscopic endophytic fungal of the six isolates showed a greenish-colored colony, white gray, clear black. Microscopic profile of each fungal isolate having spores, sprangiosphora, sporangium, conidia, hyphae and stolon. The identified secondary metabolites are: alkaloids, terpenoids, and flavonoids, and polyphenols.
References
Katno, Pramono S. 2008. Level Benefits andSafety of Traditional Medicinal Plants. Faculty of Pharmacy UGM; Jogjakarta.
Isolation and Characterization Endophytic Fungal Isolate from Peronema canescensJack Leaf and Coptosapelta tomentosaVal. K. Heyne Root J. Trop. Pharm. Chem. 2019. Vol 4. No. 5.p-ISSN: 2087-7099; e-ISSN: 2407-6090224
Strobel, G.A. dan B. Daisy. 2003. Bioprospecting for Microbial Endophytes and Their Natural Products. Microbiology and Molecular Biology Reviews. Volume 67 Nomor 4: hal 491-502.
Tan, RX and WX Zou. 2001. Endophytes: a rich source of functional metabolites.Nat Prod. Rep.18: 448-459.
Tombe, M. 2008. Fungal Endofit As An Antibiotics Producer. C.V. Meori Agro; Jakarta[5]Setyowati, M.F., 2010. Ethnofarmakologiand Usage of Medicinal Plants of Dayak Tunjung In East Kalimantan, Health R & D Media, XX (3). Avalaiblefrom: https://scholar.google.com.
Al'amri, A.F., Herman., Amir, M. (2011). Antioxidant Activity of Merchant Root (Coptosapelta Tomentosa Valeton K. Heyne) Against DPPHFree Radicals (1,1 Diphenyl-2-Pikril Hydrazil). Bachelor's Thesis. Facultyof Pharmacy Mulawarman University.
Kitagawa, I., Simajuntak, P., Hori, K., Nagami, N., Mahmud, T., Shibuya, H., Kobayashi, K., 1994. Indonesian Medicinal Plants. VII. Seven New Clerodane-Type Diterpenoids, Peronemins A2, A3, B1, B2, B3, C1, And D1, From The Leaves Of Peronema Canescens (Verbenaceae). Chem. Pharm. Bull.42(5) : 1050-1055.
Harmida, H., Sarno., Yuni., V.F., 2011. EthnofitomedikaStudy In Lawang Agung Village Mulak Ulu Sub-District, Lahat Regency of South Sumatra. Journal of Science Research. 14 (1): 42-46.
Ibrahim, A. and Kuncoro, H., 2012. Identification of Secondary Metabolites and Antibacterial Activity of Sungkai Leaf Extract (Peronema Canescens Jack.) Against Some Pathogenic Bacteria. J. Trop.Pharm. Chem. 2 (1): 8-18
Ramadenti, F., Sundaryono, A., Handayani, D., 2017. Ethyl Acetate Fraction Test Leaves Peresema Canescens Against Plasmodium Berghei At Mus Musculus. Alotrop Journal of Education and Chemistry. 1 (2): 89-92.
Rosdiana, N.A., 2014. Active Antioxidant FactionOf Sungkai Wood Skin Extract (Peronema Canescens Jack.). Bogor Agricultural Institute.[12]Ahmad, I., Ibrahim, A., 2015. Bioactivity Methanol Extract And N-Hexane Faction of Sungkai Leaf (P. Canescens Jack) Against Shrimp Larvae (A. Salina Leach). Journal of Science and Health. 1 (3): 114-119.
Shella, A. 2011. Identification of Secondary Metabolites and Bio-Effectiveness Test of Rooted Roots (C. tomentosa Valton K. Heyne) Against Artemia Salina Leach. Bachelor's Thesis. Faculty of Pharmacy Mulawarman University. Samarinda.[14]Kumala, Shirly, Erlita A., andPriyo Wahyudi. 2007. Activity of Secondary Metabolite Antimicrobial Capang Endophytic Trengguli Plant (Cassia Fistula L). Journal Alam IndonesiaVol. 6 No. 2: 46 -48.
Gandjar, I., W. Sjamsuridzal, and A. Oetari. 2006. Basic and Applied Psychology. Yayasan Obor Indonesia; Jakarta.
Agusta, A. 2009. Biological and Chemical Fungal Endofit. ITB Publishers; Bandung.
Djide, N. M. and Sartini. 2008. Analysis of Pharmaceutical Microbiology. Microbiology Laboratory of Department of PharmacyUNHAS; Macassar.
Jawetz, E., J. L. Melnick, and Adelberg. 2005. Microbiology For Health Professions. Translated by Isolation and Characterization Endophytic Fungal Isolate from Peronema canescensJack Leaf and Coptosapelta tomentosaVal. K. Heyne Root J. Trop. Pharm. Chem. 2019. Vol 4. No. 5.p-ISSN: 2087-7099; e-ISSN: 2407-6090225Huriati and Hartanto. EGC Publisher; Jakarta.
Radji, M. 2005. The Role of Biotechnology and Endophytic Microbes in the Development of Herbal Medicines. Pharmaceutical Science Magazine. Volume 2 No. 3: pp. 113-126.
Carlile, M.J., S.C. Watkinson, and G.W. Gooday. 2001. The fungal. Academic Press; California
Rohman, A. 2007. Pharmaceutical Chemical Analysis. Student Library; Yogyakarta.
