Optimized Ethyl Acetate Extraction of Mangifera casturi Leaves: Impact of Temperature and Extraction Time on TPC, TFC, and Antioxidant Activity
DOI:
https://doi.org/10.30872/jtpc.v10i2.411Keywords:
Casturi Mango, DPPH method, TPC, TFC, Optimized extractionAbstract
Mangifera casturi is an endemic plant from South Kalimantan that has been traditionally used for its medicinal properties. This study aimed to determine the optimal extraction conditions of Mangifera casturi leaves ethyl acetate extract and evaluate its total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity. Extraction was performed using maceration assisted by an ultrasonic water bath at temperatures of 30, 40, and 50°C with extraction times of 30, 60, and 90 minutes. TPC was determined using the Folin-Ciocalteu method, TFC was measured using the AlCl₃ colorimetric method, and antioxidant activity was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay. The optimal condition for TPC was achieved at 40°C for 90 minutes, yielding 89.09 ± 12.385 mg GAE/g extract. The optimal condition for TFC was obtained at 40°C for 30 minutes, yielding 86.68 ± 1.987 mg QE/g extract. The optimal antioxidant activity was obtained at 30°C for 90 minutes, with an IC₅₀ value of 63.869 ± 0.431 ppm, indicating strong antioxidant activity. These findings suggest that extraction temperature and time significantly influence the phytochemical content and antioxidant potential of M. casturi leaves ethyl acetate extract, and that this plant represents a promising source of natural antioxidants.
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[1] Hanin, N.N.F., & Pratiwi, R., 2017. Kandungan fenolik, flavonoid dan aktivitas antioksidan ekstrak daun paku laut (Acrostichum aureum L.) fertil dan steril di kawasan mangrove Kulon Progo, Yogyakarta, Journal of Tropical Biodiversity and Biotechnology, 2, (2), 51–57.
[2] Rauf, A., Ahmad, Z., Formanowicz, D., Ribaudo, G., & Alomar, T.S., 2024. Antioxidant potential of polyphenolic and flavonoid compounds, Frontiers in Chemistry, 12, 1463755. https://doi.org/10.3389/fchem.2024.1463755
[3] Marliani, L., Naimah, A., & Roni, A., 2016. Penetapan kadar fenolat total dan flavonoid total ekstrak etanol daun, kulit batang dan kulit buah kasturi (Mangifera casturi), Proceeding of Mulawarman Pharmaceuticals Conferences, 3, (2), 275–281. https://doi.org/10.25026/mpc.v3i2.121
[4] Ramadhan, H., Rezky, D.P., & Susiani, E.F., 2021. Penetapan kandungan total fenolik-flavonoid pada fraksi etil asetat kulit batang kasturi (Mangifera casturi Kosterman), Jurnal Farmasi dan Ilmu Kefarmasian Indonesia, 8, (1), 58–67.
[5] Gunawan, G., Muhamat, M., & Maghfiroh, A., 2024. Ethnobotany and conservation of Kasturi (Mangifera casturi Koesterm.) by Banjar Tribe in South Kalimantan, Indonesia, Biodiversitas, 25, (4), 1420–1426. https://doi.org/10.13057/biodiv/d250414
[6] Pratama, M., Razak, R., & Rosalina, V.S., 2019. Analisis kadar tanin total ekstrak etanol bunga cengkeh (Syzygium aromaticum L.) menggunakan metode spektrofotometri UV-Vis, Jurnal Fitofarmaka Indonesia, 6, (2), 368–373.
[7] Kchaou, W., Abbès, F., Blecker, C., Attia, H., & Besbes, S., 2013. Effects of extraction solvents on phenolic contents and antioxidant activities of Tunisian date varieties (Phoenix dactylifera L.), Industrial Crops and Products, 45, 262–269. https://doi.org/10.1016/j.indcrop.2012.12.047
[8] Palaiogiannis, D., Karavasiloglou, N., Ntourtoglou, G., & Irakli, M., 2023. Successive solvent extraction of polyphenols and antioxidant compounds using solvents of increasing polarity, Chemical Proceedings, 3, (3), 18. https://doi.org/10.3390/chemproc2023018018
[9] Rafiee, Z., Jafari, S.M., Alami, M., & Khomeiri, M., 2011. Microwave-assisted extraction of phenolic compounds from olive leaves, Food Chemistry, 129, (3), 1150–1157. https://doi.org/10.1016/j.foodchem.2011.05.046
[10] Assefa, A.D., Jeong, Y.J., Kim, D.J., Jeon, Y.A., Lee, J.E., Lee, H.S., et al., 2016. Antioxidant and antiplatelet activities of flavonoid-rich fractions of citrus fruits from Korea, 3 Biotech, 6, (1), 1–13. https://doi.org/10.1007/s13205-015-0348-4
[11] Ghafoor, K., & Choi, Y.H., 2009. Optimization of ultrasound assisted extraction of phenolic compounds and antioxidants from grape peel through response surface methodology, Journal of the Korean Society for Applied Biological Chemistry, 52, (3), 295–300. https://doi.org/10.3839/jksabc.2009.050
[12] Sanou, A., Dabiré, C., Dicko, M.H., et al., 2023. Modelling and optimisation of ultrasound-assisted extraction of phenolic compounds and antioxidant activity, Scientific Reports, 13, 1149. https://doi.org/10.1038/s41598-023-28183-4
[13] Altemimi, A., Choudhary, R., Watson, D.G., & Lightfoot, D.A., 2016. Ultrasound assisted extraction of phenolic compounds from peaches and pumpkins, PLoS ONE, 11, (2), e0148758. https://doi.org/10.1371/journal.pone.0148758
[14] Fuad, A.F., Alwi, A., Zulkifly, N.A.H., & Mohamed, N.A., 2020. Total phenolic, total flavonoid content and antioxidant activity of Mangifera sp. leaf extracts, Journal of Agrobiotechnology, 11, (1 Suppl.), 69–78. https://doi.org/10.37231/jab.2020.11.1S.236
[15] Mulyani, F., Rahayu, Y.P., Daulay, A.S., & Nasution, H.M., 2023. Phytochemical screening and antioxidant activity test of ethanol extract of casturi mango leaves (Mangifera casturi Koesterm.) using the DPPH method, Journal of Pharmaceutical Sciences, 6, (Suppl. 1), 49–63.
[16] Kumar, M., Saurabh, V., Tomar, M., Hasan, M., Changan, S., Sasi, M., et al., 2021. Mango (Mangifera indica L.) leaves: nutritional composition, phytochemical profile, and health-promoting bioactivities, Antioxidants, 10, (2), 299. https://doi.org/10.3390/antiox10020299
[17] Panche, A.N., Diwan, A.D., & Chandra, S.R., 2016. Flavonoids: an overview, Journal of Nutritional Science, 5, e47. https://doi.org/10.1017/jns.2016.41
[18] Dai, J., & Mumper, R.J., 2010. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties, Molecules, 15, (10), 7313–7352. https://doi.org/10.3390/molecules15107313
[19] Shahidi, F., & Ambigaipalan, P., 2015. Phenolics and polyphenolics in foods, beverages and spices: antioxidant activity and health effects, Journal of Functional Foods, 18, 820–897. https://doi.org/10.1016/j.jff.2015.06.018
[20] Medina-Torres, N., Ayora-Talavera, T., Espinosa-Andrews, H., Sánchez-Contreras, A., & Pacheco, N., 2017. Ultrasound Assisted Extraction for the Recovery of Phenolic Compounds from Vegetable Sources, Agronomy, 7, (3), 47. https://doi.org/10.3390/agronomy7030047
[21] Muflihah, Y.M., Gollavelli, G., & Ling, Y.C., 2021. Correlation study of antioxidant activity with phenolic and flavonoid compounds in Indonesian Zingiberaceae herbs, Antioxidants, 10, (9), 1365. https://doi.org/10.3390/antiox10091365
[22] Chemat, F., Rombaut, N., Sicaire, A.G., Meullemiestre, A., Fabiano-Tixier, A.S., & Abert-Vian, M., 2017. Ultrasound assisted extraction of food and natural products: mechanisms, techniques, combinations, protocols and applications, Ultrasonics Sonochemistry, 34, 540–560. https://doi.org/10.1016/j.ultsonch.2016.06.035
[23] Alara, O.R., Abdurahman, N.H., & Ukaegbu, C.I., 2021. Extraction of phenolic compounds: a review, Current Research in Food Science, 4, 200–214. https://doi.org/10.1016/j.crfs.2021.03.011
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Copyright (c) 2026 Dharmastuti Cahya Fatmarahmi, Dina Lorena, Dina Mutmainah, Rahmawati Rahmawati, Mifta Fajarwati, Sabtanti Harimurti (Author)
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