Effect of Hypercholesterolemic Patient Serum on The Viability and Characteristics of Mesenchymal Stem Cells

Dini Cahyani; Nurul  Hidayah; Dian Respati Ayu; Habibi Habibi; Yanuar Ardani

doi:10.30872/jtpc.v10i2.441

Authors

Dini Cahyani Agung Putra University Author
Nurul Hidayah Immunology Laboratory, Biotechnology, Agung Putra University, Semarang, Indonesia Author
Dian Respati Ayu Stem Cell Metabolites Laboratory, Biomedical Science, Agung Putra University, Semarang, Indonesia Author
Habibi Habibi Stem Cell and Cancer Research (SCCR) Indonesia, Semarang, Indonesia Author
Yanuar Ardani Psychosomatic and Palliative Division, Department of Internal Medicine, Kariadi General Hospital, Semarang, Indonesia Author

DOI:

https://doi.org/10.30872/jtpc.v10i2.441

Keywords:

Characteristics, Hypercholesterolemic, Mesenchymal Stem Cells,, Serum patient, Viability

Abstract

Mesenchymal Stem Cells (MSCs) have regenerative therapeutic potential, including for diseases related to
hypercholesterolemia. This study aimed to assess how serum from hypercholesterolemia patients affects the
viability and characteristics of MSCs. It also investigated changes in MSC morphology and cytokine secretion
levels of TNF-α and IL-10 after 3 days of serum treatment. The study divided into three groups: control 10%
FBS, patient serum with cholesterol <200 mg/dL (P1), and patient serum with cholesterol >300 mg/dL
(P2). MSCs were exposed serum for 3 days. Cell viability was assessed using MTT assay; morphology was
observed microscopically; cytokine levels were measured by ELISA. Statistical analysis used one-way
ANOVA with Tukey’s test for normal data and Kruskal-Wallis with Dunn’s post hoc for non-normal data.
Viability was highest in the control group, slightly reduced in P1, and significantly lowered in P2.
Morphologically, control and P1 MSCs showed spindle-shaped fibroblast-like cells with high-confluency; P2
MSCs exhibited irregular, less elongated shapes and low-confluency. TNF-α levels were elevated in P1
compared to control and increased in P2. IL-10 levels cytokine secreted by MSCs showed increased in P1
compared to control and elevated in P2. Hypercholesterolemia serum reduced MSC proliferation, influenced
morphology, and differentially affected TNF-α and IL-10 secretion.

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