The Effect of Solvent Concentration Against Specific and Non Specific Parameters of Standardization: Ethanolic Extract of Papaya Seed (Carica papaya Linn.)
DOI:
https://doi.org/10.30872/j.trop.pharm.chem.v7i2.158Keywords:
Papaya Seed, Specific, Non Specific, Standardization of extractAbstract
Papaya plants (Carica papaya L.) can be used as traditional medicine. The parts of the plant commonly used as medicine are the seeds and leaves. Assurance of the quality of the extract obtained needs to be carried out by testing specific and non-specific parameters. The aim of the study was to determine the effect of the percentage of ethanol solvent concentration on specific parameters (organoleptic, phytochemical screening, and total phenolic content) and non-specific parameters (moisture content, ash content, and specific gravity) of papaya seed extract (Carica papaya L.). This type of laboratory experimental qualitative research was done to determine the effect of the percentage of ethanol solvent concentration (70% and 95%) on specific and non-specific parameters. Specific and non-specific parameter testing is based on work procedures listed in the Indonesian Herbal Pharmacopoeia. The results of testing the specific parameters on organoleptics showed no difference in the extract obtained, which was viscous and dark brown in color with a characteristic odor. Based on qualitative phytochemical screening, 70% ethanol extract contains alkaloids, flavonoids, tannins, and saponins. However, the 95% ethanol extract was only positive for alkaloids, flavonoids, and tannins. The phenolic content test of 70% ethanol and 95% ethanol extracts was 4.31 ± 0.65 mg GAE/g and 2.69 ± 0.41 mg GAE/g, respectively (Sig 0.022 < 0.05). Extract non-specific parameter test results. The ash content test for 70% and 95% ethanol extracts was 9.5% ± 0.21% w/w and 8.5% ± 0.06% w/w (Sig 0.043 < 0.05). The respective water contents were 8.7 ± 0.11% w/w and 9.7 ± 0.26% w/w (Sig 0.046 < 0.05). The specific gravity of each obtained was 0.88 ± 0.006 % g/ml and 0.75 ± 0.006% g/ml (Sig 0.043 < 0.05). The conclusion of this study is that the concentrations of 70% ethanol and 95% ethanol in papaya seed ethanol extract have an effect on specific parameters, namely the measurement of total phenolic content. While the non-specific parameters affect the water content, total ash content, and specific gravity.
References
S. M. Ninda Kirana Jati, Agung Tri Prasetya, “Isolasi, Identifikasi, dan Uji Aktivitas Antibakteri Senyawa Alkaloid pada Daun Pepaya,” vol. 42, no. 1, pp. 1–6, 2019.
A. Qurrota and A. N. Laily, “Analisis Fitokimia Daun Pepaya ( Carica papaya L .) Di Balai Penelitian Tanaman Aneka Kacang dan Umbi , Kendalpayak , Malang The Phytochemical Analysis of Papaya Leaf ( Carica papaya L .) at The Research Center of Various Bean and Tuber Crops Kendalpayak ,” pp. 134–137, 2018.
R. Afrianti, R. Yenti, and D. Meustika, “Uji Aktifitas Analgetik Ekstrak Etanol Daun Pepaya ( Carica papaya L .) pada Mencit Putih Jantan yang di Induksi Asam Asetat 1 %,” vol. 01, no. 01, pp. 54–60, 2019.
N. Jaipah, I. Saraswati, and R. Hapsari, “Uji Efektivitas Antimikroba Ekstrak Biji Pepaya (Carica papaya L.) Terhadap Pertumbuhan Escherichia coli,” vol. 6, no. 2, pp. 947–955, 2018.
Y. Y. Andriani, I. Rahmiyani, S. Amin, and T. Lestari, “Kadar Fenol Total Ekstrak Daun dan Biji Pepaya (Carica papaya L.) Menggunakan Metode Spektrofotometri UV-VIS,” vol. 15, pp. 73–78, 2019.
S. Uv-visibel, A. K. Sari, R. Alfian, and S. Musiam, “Penetapan Kadar Fenolik Dan Flavonoid Total Ekstrak Metanol Kayu Kuning ( Arcangelisia flava Merr ) Dengan Metode,” vol. 1, no. 2, pp. 210–217, 2020.
N. Pogostemon, D. Metode, M. Tahir, A. Muflihunna, F. Farmasi, and U. M. Indonesia, “Penentuan Kadar Fenolik Total Ekstrak Etanol Daun Nilam Dengan Metode Spektrofotometri UV-VIS,” vol. 4, no. 1, pp. 215–218, 2020.
P. Riwanti, F. Izazih, and Hang, “Journal of Pharmaceutical Care Anwar Medika Artikel Penelitian Pengaruh Perbedaan Konsentrasi Etanol pada Kadar Flavonoid Total Ekstrak Etanol 50,70 dan 96%,” vol. 2, no. 2, pp. 82–95, 2020.
G. P. G. Putra, L. P. Wrasiati, F. T. Pertanian, U. Udayana, and K. Bukit, “Pengaruh Jenis Pelarut dan Waktu Maserasi terhadap Ekstrak Kulit Biji Kakao ( Theobroma cacao L .) sebagai Sumber Antioksidan,” vol. 8, no. 1, pp. 150–159, 2020.
V. K. Wardani and D. Saryanti, “Formulasi Transdermal Patch Ekstrak Etanol Biji Pepaya ( Carica Papaya L .) Dengan Basis Hydroxypropil Metilcellulose ( HPMC ),” vol. 4, no. 1, pp. 38–44, 2021, doi: 10.13057/smj.v4i1.
E. Sulastri et al., “Total Phenolic , Total Flavonoid , Quercetin Content and Antioxidant Activity of Standardized Extract of Moringa oleifera Leaf from Regions with Different Elevation,” vol. 10, no. 6, 2018.
M. P. J. R and G. Forst, “Pengukuran Parameter Spesifik Dan Non Spesifik Ekstrak Etanol Daun,” vol. 6, no. 1, pp. 1–12, 2020.
I. N. S. Tri Saptari H., Triastinurmiatiningsih1, Bina Lohita S., “Kadar Fenolik dan Aktivitas Antioksidan Ekstrak Etanol Rumput Laut Coklat (Padina australis),” Fitofarmaka, vol. 9, no. 1, pp. 1–8, 2019.
L. Aang, R. Dewantara, A. Dwi, and Y. Andayani, “Penetapan Kadar Fenolik Total Ekstrak Kacang Panjang ( Vigna unguiculata ) dengan Metode Spektrofotometri UV-Visible,” pp. 13–19, 2021.
D. Andriani and L. Murtisiwi, “Penetapan Kadar Fenolik Total Ekstrak Etanol Bunga Telang (Clitoria Ternatea L.) Dengan Spektrofotometri Uv Vis,” Cendekia J. Pharm., vol. 2, no. 1, pp. 32–38, 2018, doi: 10.31596/cjp.v2i1.15.
Galih Samodra, “Standarisasi Parameter Spesifik dan Non Spesifik Ekstrak Etanol Buah Asam Gelugur (Garcinia atroviridis Griff.),” Viva Med., vol. 11, pp. 16–26, 2019.
N. W. Martiningsih, G. Agus, B. Widana, P. Lilik, and P. Kristiyanti, “Skrining Fitokimia dan Uji Aktivitas Antioksidan Ekstrak Etanol Daun Matoa ( Pometia pinnata ) Dengan Metode DPPH,” 2018.
D. I. Sari and L. Triyasmono, “Rendemen dan Flavonoid Total Ekstrak Etanol Kulit Batang Bangkal ( Nauclea subdita ) dengan Metode Maserasi Ultrasonikasi,” vol. 04, no. 01, pp. 48–53, 2017.
P. Riwanti, F. Izazih, U. Hang, T. Surabaya, S. Indonesia, and F. Total, “Pengaruh Perbedaan Konsentrasi Etanol pada Kadar Flavonoid Total Ekstrak Etanol 50,70 dan 96% Sargassum polycystum dari Madura,” vol. 2, no. 2, pp. 35–48, 2020.
A. Fatmawati et al., “Penetapan Kadar Flavonoid Total Ekstrak Etanol Daun Kelor ( Moringa Oleifera Lam) Dengan Metode Kromatografi Lapis Tipis Densitometri 1,” 2019.
E. Pujiastuti, “Perbandingan Kadar Flavonoid Total Ekstrak Etanol 70% dan 96% Kulit Buah Naga Merah ( Hylocereus,” vol. 5, no. 1, pp. 28–43, 2021.
E. Moghimipour and S. Handali, “Saponin: Properties, Methods of Evaluation and Applications,” Annu. Res. Rev. Biol., vol. 5, no. 3, pp. 207–220, Jan. 2015, doi: 10.9734/ARRB/2015/11674.
I. Estikawati and N. Y. Lindawati, “Jurnal Farmasi Sains dan Praktis Penetapan Kadar Flavonoid Total Buah Oyong ( Luffa Acutangula ( L .) Roxb .) Dengan Metode Spektrofotometri Uv-Vis Determination Of Total Flavonoid Levels Of Ridge Gourd Fruit With Uv- Vis Spectrumfotometry Method,” vol. V, no. 2, pp. 96–105, 2019.
V. Lattanzio, “Phenolic compounds: Introduction,” Nat. Prod. Phytochem. Bot. Metab. Alkaloids, Phenolics Terpenes, pp. 1543–1580, Jan. 2013, doi: 10.1007/978-3-642-22144-6_57/COVER.
N. N. Zahra, H. Muliasari, Y. Andayani, and I. M. Sudarma, “Analisis Kadar Fenolik Total dan Aktivitas Antiradikal Bebas Madu dan Propolis Trigona sp. Asal Lombok Utara,” Anal. Anal. Environ. Chem., vol. 6, no. 1, pp. 74–82, Apr. 2021, doi: 10.23960/AEC.V6.I1.2021.P74-82.
M. Musci and S. Yao, “Optimization and validation of Folin–Ciocalteu method for the determination of total polyphenol content of Pu-erh tea,” http://dx.doi.org/10.1080/09637486.2017.1311844, vol. 68, no. 8, pp. 913–918, Nov. 2017, doi: 10.1080/09637486.2017.1311844.
H. Boussoussa, C. Hamia, A. Djeridane, M. Boudjeniba, and M. Yousfi, “Effect of different Solvent Polarity on Extraction of Phenolic Compounds from Algerian Rhanterium adpressum Flowers and their Antimicrobial and Antioxidant Activities,” Curr. Chem. Biol., vol. 8, no. 1, pp. 43–50, Nov. 2014, doi: 10.2174/221279680801141112095950.
Anonim, “Farmakope Herbal Indonesia Edisi II Tahun 2017.” https://farmalkes.kemkes.go.id/2020/08/farmakope-herbal-indonesia-edisi-ii-tahun-2017-3/ (accessed Apr. 11, 2023).
K. RI, “Farmakope Herbal Indonesia Edisi II,” 2017.
A. S. M. Ulfa, E. Emelda, M. A. Munir, and N. Sulistyani, “Pengaruh Metode Ekstraksi Maserasi dan Sokletasi Terhadap Standarisasi Parameter Spesifik dan Non Spesifik Ekstrak Etanol Biji Pepaya (Carica papaya L.),” J. Insa. Farm. Indones., vol. 6, no. 1, pp. 1–12, May 2023, doi: 10.36387/JIFI.V6I1.1387.
M. Koenigii, T. Azis, S. Febrizky, and A. D. Mario, “Yield Alkaloid dari Daun Salam India,” vol. 20, no. 2, pp. 1–6, 2014.
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