Hidrolisis Protein Tanduk Muda Rusa Sambar (Rusa unicolor) serta Potensinya sebagai Penurun Resiko Hipertensi
Keywords:
Tanduk muda rusa Sambar, Hidrolisis protein, Hipertensi, Angiotensin converting enzyme inhibitorAbstract
Penelitian ini bertujuan untuk mengetahui tingkat hidrolisis tanduk muda rusa Sambar (Rusa unicolor) dengan enzim pencernaanm yaitu enzim pepsin dan tripsin, serta mengetahui adanya aktivitas Angiotensin I-Converting Enzyme Inhibitor dari protein tanduk muda tersebut. Protein tanduk muda rusa Sambar dihidrolisis dengan enzim pepsin dan tripsin selanjutnya diuji aktivitas penghambatan ACE (ACE-I). Hasil dari penelitian menunjukkan bahwa tanduk muda rusa Sambar mengandung air 11,34 %; protein sebesar 22,4%; lemak 13,74% dan abu sebesar 37,29%. Potensi sebagai agen antihipertensi dilakukan uji penghambatan angiotensin converting enzyme (ACE). Berdasarkan hasil penelitian diketahui adanya aktivitas penghambat ACE sebesar 83,38 % pada konsentrasi protein 1,266 mg/ml.
Downloads
References
1.AOAC.1998. Official Methods of Analysis of AOAC International. Association of Official Analysis Chemists International. doi:10.3109/15563657608988149
2.Bunnel, T. 1999. The Effect of “Healing with Intent†on Pepsin Enzyme Activity. J. Sci. Exp. 13( 2) : 139–148.
3.Cheng, F.-Y., Liu, Y.-T., Wan, T.-C., Lin, L.-C., & Sakata, R. 2008. The development of angiotensin I-converting enzyme inhibitor derived
from chicken bone protein. Animal Science Journal, 79(1), 122–128. doi:10.1111/j.1740-0929.2007.00507
4.Cheung, H., F. Wang, & M.A. Ondetti. 1980. Binding of Peptide Substrates and Inhibitors, 255(2), 401–405.
5.Fujita, H., K. Yokoyama, and M. Yoshikawa. 2000. Classification and antihypertensive activity of angiotensin I-converting enzyme inhibitory peptides derived from food proteins. Journal of Food Science.Vol. 65: 564-594.
6.Hernández-Ledesma, B., M. M Contreras, I. Recio. 2011. Antihypertensive peptides: production, bioavailability and incorporation into foods.Advances in Colloid and Interface Science. Vol. 165: 23–35
7.Jamal, Y., G. Semiadi dan R.T.P Nugraha. 2005. Kualitas produk ranggah mudarusa sambartangkaran. Berkala Ilmiah Biologi (UGM) 4:325-336.
8.Jang, A , & M. Lee. 2005. Purification and identification of angiotensin converting enzyme inhibitory peptides from beef hydrolysates. Meat Science, 69(4), 653–61. doi:10.1016/j.meatsci.2004.10.014
9.Katayama, T., K. Katayama, Jamhari, T, Mori, S. Kawahara, K. Miake, Y. Kodama, M. Sugiyama, Y. Kawamura, T. Takayama, M.
Maruyama, M. Muguruma. 2007. Angiotensin I-converting enzyme inhibitor peptide derived from procine skeletal muscle myosin and
it’s antihypertensive activity in spontaneously hypertensive rats.
Journal of Food Science. Vol. 72: S702-S706.
10.Matsui, T., H. Matsufuji, E. Seki, K. Osajima, M. Nakashima, and Y. Osajima. 1993. Inhibition of angiotensin I-converting enzyme by bacillus licheniformis alkaline protease hydrolyzates derived from sardine muscle. Bioscience Biotechnology Biochemical. Vol. 57(6): 922-925.
11.Murray, B. A., and RR. J. Fitzgerard. 2007. Angiotensin Converting EnzymePeptides Derived from Food Protein:Biochemistry,Bioactivity,
And Production. Current Pharmaceutical Design. Vol. 13: 773-791.
12.Roberts, PR,. Burney JD, Black KW, Zaloga GP. 1999. Effect of chain length on absorption of biologically active peptides from the
gastrointestinal tract digestion. International Journal of Gastroenterology. Vol. 60:332–337.
13.Saiga, A., Okumura, T., Makihara, T., Katsuda, S.-I., Morimatsu,F., & Nishimura, T. 2006. Action mechanism of an angiotensin I-converting enzyme inhibitory peptide derived from chicken breast muscle. Journal of Agricultural and Food Chemistry, 54, 942–945. doi:10.1021/jf0508201
14.Saiga, A., T. Okumura, T. Makihara, S. Katsuda, F. Morimatsu, and T. Nishimura, 2006. Action mechanism of an angiotensin I-converting
enzyme inhibitory peptide derived from chicken breast muscle. J. Agr.
Food Chem. 54: 942-945.
15.Semiadi. 2001. Potensi pengembangan peternakan rusa sambar di Kabupaten Paser. Samarinda.
16.Suhermiyati, S.. S. J. Setiyawati. 2008. Potensi limbah nanas untuk peningkatan kualitas limbah lkan tongkol sebagai bahan pakan unggas. J. Anim. Prod. 10, (3) : 174 –178.
17.Susalit,E., E.J Kapojos, dan H.R Lubis. 2001. Buku Ajar Ilmu
Penyakit Dalam: Hipertensi Primer. Balai Penerbit FKUI. Jakarta
18.Yamamoto, N., A. Akino, T. Takano. 1994. Antihypertensive effect of the peptides derived from casein by an extracellular proteinase from
Lactobacillus helveticusCP790. Journal of Dairy Science.Vol. 77: 917–922.
19.Yokoyama, K.; H. Chiba.; M. Yoshikawa. 1992. Peptide inhibitors
for angiotensin I-converting enzyme from a thermolysin digest of dried
bonito. Bioscience, Biotechnology, and Biochemistry. Vol. 56:1541–
1545.
