The Potential Of Citronella (Cymbopogon Nardus (L.) Rendle) Essential Oil In Inhibiting The Formation Of Escherichia Coli And Candida Albicans Biofilms
DOI:
https://doi.org/10.30872/jtpc.v9i2.303Keywords:
Biofilm , Citronella, E. coli, C. albicansAbstract
Indonesia is a tropical country that has very high biodiversity, one of which is the citronella plant (Cymbopogon nardus (L.) Rendle). Citronella leaves have a strong distinctive aroma and essential oils are odorous compounds found in the plant. This study aims to identify the components of compounds in essential oils in citronella oil and the potential for antibiofilm against Escherichia coli and Candida albicans. Essential oils were obtained through a distillation process. Furthermore, they were tested in four concentration series (0.125%; 0.25%; 0.5%; 0.1%) to measure the activity of the ability to inhibit the formation of E. coli and C. albicans biofilms using the microdilution method. The results of the antibiofilm test were analyzed based on optical density to calculate the percentage of inhibition. Citronella essential oil with the highest concentration of 1% showed inhibition of biofilm formation against E. coli and C. albicans of 84% and 79% respectively. Citronella essential oil can inhibit the formation of E. coli and C. albicans biofilms.
Downloads
References
[1] P. A. de S. Rolim et al., “Antifungal, Antioxidant, and Irritative Potential of Citronella Oil (Cymbopogon nardus) Associated with Phenethyl Ester of Caffeic Acid (CAPE),” Cosmetics, vol. 11, no. 5, 2024, doi: 10.3390/cosmetics11050162.
[2] M. F. C. S. &Luciano M. Priscilla Moreira Curtis Peixoto, Armanda Aparecida Júlio, Ester Gonçalves de Jesus, Aldino Neto Venancio, Luciana Alves Parreira, “Fungicide potential of citronella and tea tree essential oils against tomato cultivation’s phytopathogenic fungus Fusarium oxysporum f. sp. lycopersici and analysis of their chemical composition by GC/MS,” Nat. Prod. Res., vol. 38, no. 4, 2024.
[3] A. C. S. &Ramesh K. S. Ankita Maurya, Himanshu Yadav, Richa Sharma, Anoop Kumar Verma, Durga Prasad Mindala, “No Antifungal efficacy and phytochemical characterization of citronella (Cymbopogon winterianus Jowitt ex Bor) essential oil against fungal pathogens causing severe diseases in Mentha arvensis L.Title,” J. Essent. Oil Bear. Plants, vol. 27, 2024.
[4] S. Dangol et al., “Essential Oil Composition Analysis of Cymbopogon Species from Eastern Nepal by GC-MS and Chiral GC-MS, and Antimicrobial Activity of Some Major Compounds,” Molecules, vol. 28, no. 2, 2023, doi: 10.3390/molecules28020543.
[5] M. O. P. 1 Andreia Patrícia Magalhães 1, Paula Jorge 1, “Pseudomonas aeruginosa and Staphylococcus aureus communication in biofilm infections: insights through network and database construction,” Crit. Rev. Microbiol., vol. 45, no. 5–6, 2019.
[6] H. Hamzah, U. Muhammadiyah, K. Timur, T. Hertiani, and U. G. Mada, “Efficacy Of C-10 Massoialactone against-Multispecies Microbial Biofilm,” no. June, 2022, doi: 10.33263/BRIAC123.34723487.
[7] M. Bafadal, T. Hertiani, S. U. T. Pratiwi, and A. C. Narsa, “Exploring The Inhibitory Potential of Massoia Oil on Biofilm Dual-Species Culture of Staphylococcus aureus and Pseudomonas aeruginosa,” Egypt. J. Chem., vol. 66, no. 8, pp. 185–190, 2023, doi: 10.21608/ejchem.2022.169290.7100.
[8] Y. Luo and Y. Song, “Mechanism of antimicrobial peptides: Antimicrobial, anti-inflammatory and antibiofilm activities,” Int. J. Mol. Sci., vol. 22, no. 21, 2021, doi: 10.3390/ijms222111401.
[9] H. Hamzah, T. Hertiani, S. Utami, T. Pratiwi, and T. Nuryastuti, “The bio ilm eradication activity of C-10 massoialactone against Staphylococcus aureus,” Int. J. Res. Pharm. Sci., no. October, 2020, doi: 10.26452/ijrps.v11i4.3228.
[10] H. Hamzah, U. Muhammadiyah, K. Timur, T. Hertiani, and U. G. Mada, “Efek Saponin Terhadap Penghambatan Planktonik Dan Mono-Spesies Biofilm Candida albicans ATCC 10231 Pada Fase Pertengahan , Pematangan Dan Efek Saponin Terhadap Penghambatan Planktonik Dan Mono-Spesies Biofilm Candida albicans ATCC 10231 Pada Fase Pertenga,” no. May, 2021, doi: 10.22146/farmaseutik.v17i2.54444.
[11] L. A. Trindade et al., “The antifungal and antibiofilm activity of Cymbopogon nardus essential oil and citronellal on clinical strains of Candida albicans,” Brazilian J. Microbiol., pp. 1231–1240, 2022, doi: 10.1007/s42770-022-00740-2.
[12] L. S. Wei and W. Wee, “Chemical composition and antimicrobial activity of Cymbopogon nardus citronella essential oil against systemic bacteria of aquatic animals,” Iran. J. Microbiol., vol. 5, no. 2, pp. 147–152, 2013.
[13] M. Mukarram et al., “Lemongrass essential oil components with antimicrobial and anticancer activities,” Antioxidants, vol. 11, no. 1, pp. 1–23, 2022, doi: 10.3390/antiox11010020.
[14] A. Activities, D. Dantas, D. O. Alencar, E. L. De Souza, and E. Thayse, “Microencapsulation of Cymbopogon citratus D.C. Stapf Essential Oil with Spray Drying: Development, Characterization, and Antioxidant and Antibacterial Activities,” 2022.
[15] L. F. De Paiva, A. B. A. Teixeira-Loyola, T. B. Schnaider, A. C. De Souza, L. M. Zacaroni Lima, and D. R. Dias, “Association of the essential oil of Cymbopogon citratus (DC) Stapf with nystatin against oral cavity yeasts,” An. Acad. Bras. Cienc., vol. 94, no. 1, pp. 1–13, 2022, doi: 10.1590/0001-3765202220200681.
[16] S. G. and R. R. R. Dibyajit Lahiri, Moupriya Nag, “The Chemistry of Antibiofilm Phytocompounds,” Mini-Reviews Med. Chem., vol. 21, no. 9, 2021.
[17] F. Ben Abdallah, R. Lagha, and A. Gaber, “Biofilm inhibition and eradication properties of medicinal plant essential oils against methicillin-resistant staphylococcus aureus clinical isolates,” Pharmaceuticals, vol. 13, no. 11, pp. 1–15, 2020, doi: 10.3390/ph13110369.
[18] N. F. B. Sharifi A, “Selected plant essential oils inhibit biofilm formation and luxS- and pfs-mediated quorum sensing by Escherichia coli O157:H7.,” Lett Appl Microbiol, 2022.
[19] M. Khosakueng, S. Taweechaisupapong, W. Boonyanugomol, P. Prapatpong, S. Wongkaewkhiaw, and S. Kanthawong, “Cymbopogon citratus L. essential oil as a potential anti-biofilm agent active against antibiotic-resistant bacteria isolated from chronic rhinosinusitis patients,” Biofouling, vol. 40, no. 1, pp. 26–39, 2024, doi: 10.1080/08927014.2024.2305387.
[20] W. R. Li, T. H. Zeng, Z. Q. Zhang, Q. S. Shi, and X. B. Xie, “Geraniol attenuates virulence factors by inhibiting quorum sensing of Pseudomonas aeruginosa,” Front. Microbiol., vol. 14, no. April, pp. 1–10, 2023, doi: 10.3389/fmicb.2023.1190619.
[21] W. Rhimi et al., “Antifungal, Antioxidant and Antibiofilm Activities of Essential Oils of Cymbopogon spp.,” Antibiotics, vol. 11, no. 6, pp. 1–13, 2022, doi: 10.3390/antibiotics11060829.
Downloads
Published
Versions
- 2025-11-16 (2)
- 2025-11-16 (1)
Issue
Section
License
Copyright (c) 2025 Mentarry Bafadal, Hasyrul Hamzah, Supriatno Salam, Fajar Prasetya (Author)

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

